Amperometric tyrosinase biosensor based on carbon nanotube-doped sol-gel-derived zinc oxide-Nafion composite films

Joong Min Lee; Guang Ri Xu; Byung Kun Kim; Han Nim Choi; Won Yong Lee

doi:10.1002/elan.201000556

Amperometric tyrosinase biosensor based on carbon nanotube-doped sol-gel-derived zinc oxide-Nafion composite films

Joong Min Lee, Guang Ri Xu, Byung Kun Kim, Han Nim Choi, Won Yong Lee

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)-ZnO-Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17nm) of the composite film, the biosensor exhibits very fast response time of 2s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7×10^-8M (S/N=3) for phenol. The biosensor retains 88% of its initial activity after 2 weeks of storage in 50mM phosphate buffer at pH7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.

Original language	English
Pages (from-to)	962-970
Number of pages	9
Journal	Electroanalysis
Volume	23
Issue number	4
DOIs	https://doi.org/10.1002/elan.201000556
Publication status	Published - 2011 Apr

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Electrochemistry

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title = "Amperometric tyrosinase biosensor based on carbon nanotube-doped sol-gel-derived zinc oxide-Nafion composite films",

abstract = "A highly sensitive amperometric tyrosinase biosensor has been developed based on mesoporous composite films of carbon nanotube (CNT)-ZnO-Nafion composite film encapsulating tyrosinase on a glassy carbon electrode. Due to the mesoporous nature (diameter: 3.17nm) of the composite film, the biosensor exhibits very fast response time of 2s. The present biosensor shows an excellent sensitivity of 766 mA/M and a detection limit of 4.7×10-8M (S/N=3) for phenol. The biosensor retains 88% of its initial activity after 2 weeks of storage in 50mM phosphate buffer at pH7.0. The present biosensor exhibits good recovery for the spiked phenol in an industrial waste water sample.",

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AU - Choi, Han Nim

AU - Lee, Won Yong

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Amperometric tyrosinase biosensor based on carbon nanotube-doped sol-gel-derived zinc oxide-Nafion composite films

Abstract

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