The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe 2O 4 nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe 2O 4 nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.
|Number of pages||9|
|Publication status||Published - 2011 Sept|
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) ( 2010-0020587 ) and a grantof the Korea Healthcare technology R&D Project, Ministry for Health & Welfare Affairs, Republic of Korea ( A085136 ).
All Science Journal Classification (ASJC) codes
- Ceramics and Composites
- Mechanics of Materials