Abstract
Purpose: Cockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease. Methods: A cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA. Results: The deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1% identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6%) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract. Conclusions: A novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6.
| Original language | English |
|---|---|
| Pages (from-to) | 283-289 |
| Number of pages | 7 |
| Journal | Allergy, Asthma and Immunology Research |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2015 |
Bibliographical note
Publisher Copyright:© The Korean Academy of Asthma, Allergy and Clinical Immunology.
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
- Pulmonary and Respiratory Medicine