Agromyces soli sp. nov., isolated from farm soil

A Gram-positive, aerobic to microaerophilic, non-motile bacterial strain, designated MJ21 ^T, was isolated from farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain MJ21 ^T was placed within the genus Agromyces, and exhibited relatively high levels of similarity to Agromyces ulmi XIL01 ^T (97.8 %), Agromyces aurantiacus YIM 21741 ^T (97.1 %), Agromyces mediolanus JCM 3346 ^T (96.7 %), A. mediolanus JCM 1376 (99.1 %), A. mediolanus JCM 9632 (99.1 %), A. mediolanus JCM 9633 (98.9 %) and A. mediolanus JCM 9631 (96.5 %). Chemotaxonomic data also supported the classification of strain MJ21 ^T within the genus Agromyces. The new isolate contained MK-12 as the predominant menaquinone and rhamnose, galactose and xylose as cell-wall sugars. The major cellular fatty acids (>10 % of the total) were anteiso-C _{15: 0}, anteiso-C _{17: 0} and iso-C _{16: 0}. Cell-wall amino acids were 2,4-diaminobutyric acid, glutamic acid, glycine and alanine. Diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and one unidentified phospholipid were detected as polar lipids. The DNA G+C content of strain MJ21 ^T was 73.4 mol%. However, levels of DNA-DNA relatedness between strain MJ21 ^T and the seven phylogenetically closest Agromyces strains ranged from 14 to 56 %, showing clearly that the new isolate represents a novel genomic species. Strain MJ21 ^T could be differentiated clearly from its phylogenetic neighbours on the basis of phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ21 ^T is considered to represent a novel species of the genus Agromyces, for which the name Agromyces soli sp. nov. is proposed. The type strain is MJ21 ^T (= KCTC 19549 ^T = JCM 16247 ^T).