TY - JOUR
T1 - Advances in the Chromosome-Centric Human Proteome Project
T2 - looking to the future
AU - Paik, Young Ki
AU - Omenn, Gilbert S.
AU - Hancock, William S.
AU - Lane, Lydie
AU - Overall, Christopher M.
N1 - Publisher Copyright:
© 2017 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2017/12/2
Y1 - 2017/12/2
N2 - Introduction: The mission of the Chromosome-Centric Human Proteome Project (C-HPP), is to map and annotate the entire predicted human protein set (~20,000 proteins) encoded by each chromosome. The initial steps of the project are focused on ‘missing proteins (MPs)’, which lacked documented evidence for existence at protein level. In addition to remaining 2,579 MPs, we also target those annotated proteins having unknown functions, uPE1 proteins, alternative splice isoforms and post-translational modifications. We also consider how to investigate various protein functions involved in cis-regulatory phenomena, amplicons lncRNAs and smORFs. Areas covered: We will cover the scope, historic background, progress, challenges and future prospects of C-HPP. This review also addresses the question of how we can best improve the methodological approaches, select the optimal biological samples, and recommend stringent protocols for the identification and characterization of MPs. A new strategy for functional analysis of some of those annotated proteins having unknown function will also be discussed. Expert commentary: If the project moves well by reshaping the original goals, the current working modules and team work in the proposed extended planning period, it is anticipated that a progressively more detailed draft of an accurate chromosome-based proteome map will become available with functional information.
AB - Introduction: The mission of the Chromosome-Centric Human Proteome Project (C-HPP), is to map and annotate the entire predicted human protein set (~20,000 proteins) encoded by each chromosome. The initial steps of the project are focused on ‘missing proteins (MPs)’, which lacked documented evidence for existence at protein level. In addition to remaining 2,579 MPs, we also target those annotated proteins having unknown functions, uPE1 proteins, alternative splice isoforms and post-translational modifications. We also consider how to investigate various protein functions involved in cis-regulatory phenomena, amplicons lncRNAs and smORFs. Areas covered: We will cover the scope, historic background, progress, challenges and future prospects of C-HPP. This review also addresses the question of how we can best improve the methodological approaches, select the optimal biological samples, and recommend stringent protocols for the identification and characterization of MPs. A new strategy for functional analysis of some of those annotated proteins having unknown function will also be discussed. Expert commentary: If the project moves well by reshaping the original goals, the current working modules and team work in the proposed extended planning period, it is anticipated that a progressively more detailed draft of an accurate chromosome-based proteome map will become available with functional information.
KW - Chromosome-Centric Human Proteome Project
KW - HUPO
KW - SRMAtlas
KW - functional annotation
KW - human protein atlas
KW - long non-coding RNA
KW - mass spectrometry
KW - missing proteins
KW - neXtProt
KW - peptideatlas
KW - protein evidence
KW - proteomexchange
KW - smORF
KW - targeted proteomics
UR - http://www.scopus.com/inward/record.url?scp=85034852894&partnerID=8YFLogxK
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U2 - 10.1080/14789450.2017.1394189
DO - 10.1080/14789450.2017.1394189
M3 - Review article
C2 - 29039980
AN - SCOPUS:85034852894
SN - 1478-9450
VL - 14
SP - 1059
EP - 1071
JO - Expert Review of Proteomics
JF - Expert Review of Proteomics
IS - 12
ER -