Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing Acinetobacter baumannii

Thao Nguyen Vu; Jung Hyun Byun; Roshan D’Souza; Naina Adren Pinto; Le Phuong Nguyen; Dongeun Yong; Yunsop Chong

doi:10.3343/alm.2020.40.1.21

Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing Acinetobacter baumannii

Thao Nguyen Vu, Jung Hyun Byun, Roshan D’Souza, Naina Adren Pinto, Le Phuong Nguyen, Dongeun Yong, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Background: The existing modified carbapenem inactivation methods (mCIMs) recommended by the CLSI for detecting carbapenemase production have not been applicable for Acinetobacter baumannii. We evaluated the influence of matrices used in mCIMs and CIMTris on the stability of the disks for detecting carbapenemase producers and suggested optimal mCIM conditions for detecting carbapenemase-producing A. baumannii. Methods: Seventy-three A. baumannii isolates characterized for antimicrobial susceptibility and carbapenemase encoding genes were tested for carbapenemase production using mCIM and CIMTris. The influence of the matrices (Tryptic soy broth [TSB] and Tris-HCl) used in these methods on the stability of the meropenem (MEM) disk was also evaluated. The mCIM conditions were adjusted to enhance screening sensitivity and specificity for detecting carbapenemase-producing A. baumannii. Results: The matrices had an impact on the stability of the MEM disk after the incubation period (two or four hrs). TSB nutrient broth is an appropriate matrix for mCIM compared with Tris-HCl pH 7.6, which leads to the loss of MEM activity in CIMTris. The sensitivity and the specificity of the optimal mCIM were both 100%. Conclusions: We established optimal mCIM conditions for simple, accurate, and reproducible detection of carbapenemase-producing A. baumannii.

Original language	English
Pages (from-to)	21-26
Number of pages	6
Journal	Annals of laboratory medicine
Volume	40
Issue number	1
DOIs	https://doi.org/10.3343/alm.2020.40.1.21
Publication status	Published - 2020

Bibliographical note

Funding Information:
This work was supported by the Nano Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2017M3A7B4039936); the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Korea (grant No. HI17C1807); and the BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as a Global Frontier Project (H-GUARD_2014-M3A6B2060509). This work was also supported by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University.

Funding Information:
This work was supported by the Nano Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2017M3A7B4039936); the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Korea (grant No. HI17C1807); and the BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as a Global Frontier Project (H-GUARD_2014-M3A6B2060509).

Funding Information:
This work was also supported by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University.

Publisher Copyright:
© Korean Society for Laboratory Medicine

All Science Journal Classification (ASJC) codes

Biochemistry, medical
Clinical Biochemistry

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10.3343/alm.2020.40.1.21

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@article{d4730ef25e184ae08f558cbc4adaa3cb,

title = "Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing Acinetobacter baumannii",

abstract = "Background: The existing modified carbapenem inactivation methods (mCIMs) recommended by the CLSI for detecting carbapenemase production have not been applicable for Acinetobacter baumannii. We evaluated the influence of matrices used in mCIMs and CIMTris on the stability of the disks for detecting carbapenemase producers and suggested optimal mCIM conditions for detecting carbapenemase-producing A. baumannii. Methods: Seventy-three A. baumannii isolates characterized for antimicrobial susceptibility and carbapenemase encoding genes were tested for carbapenemase production using mCIM and CIMTris. The influence of the matrices (Tryptic soy broth [TSB] and Tris-HCl) used in these methods on the stability of the meropenem (MEM) disk was also evaluated. The mCIM conditions were adjusted to enhance screening sensitivity and specificity for detecting carbapenemase-producing A. baumannii. Results: The matrices had an impact on the stability of the MEM disk after the incubation period (two or four hrs). TSB nutrient broth is an appropriate matrix for mCIM compared with Tris-HCl pH 7.6, which leads to the loss of MEM activity in CIMTris. The sensitivity and the specificity of the optimal mCIM were both 100%. Conclusions: We established optimal mCIM conditions for simple, accurate, and reproducible detection of carbapenemase-producing A. baumannii.",

author = "{Nguyen Vu}, Thao and Byun, {Jung Hyun} and Roshan D{\textquoteright}Souza and Pinto, {Naina Adren} and Nguyen, {Le Phuong} and Dongeun Yong and Yunsop Chong",

note = "Funding Information: This work was supported by the Nano Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2017M3A7B4039936); the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Korea (grant No. HI17C1807); and the BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as a Global Frontier Project (H-GUARD_2014-M3A6B2060509). This work was also supported by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University. Funding Information: This work was supported by the Nano Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2017M3A7B4039936); the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Korea (grant No. HI17C1807); and the BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as a Global Frontier Project (H-GUARD_2014-M3A6B2060509). Funding Information: This work was also supported by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University. Publisher Copyright: {\textcopyright} Korean Society for Laboratory Medicine",

year = "2020",

doi = "10.3343/alm.2020.40.1.21",

language = "English",

volume = "40",

pages = "21--26",

journal = "Annals of laboratory medicine",

issn = "2234-3806",

publisher = "Seoul National University",

number = "1",

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TY - JOUR

T1 - Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing Acinetobacter baumannii

AU - Nguyen Vu, Thao

AU - Byun, Jung Hyun

AU - D’Souza, Roshan

AU - Pinto, Naina Adren

AU - Nguyen, Le Phuong

AU - Yong, Dongeun

AU - Chong, Yunsop

N1 - Funding Information: This work was supported by the Nano Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2017M3A7B4039936); the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Korea (grant No. HI17C1807); and the BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as a Global Frontier Project (H-GUARD_2014-M3A6B2060509). This work was also supported by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University. Funding Information: This work was supported by the Nano Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (No. 2017M3A7B4039936); the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Korea (grant No. HI17C1807); and the BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as a Global Frontier Project (H-GUARD_2014-M3A6B2060509). Funding Information: This work was also supported by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University. Publisher Copyright: © Korean Society for Laboratory Medicine

PY - 2020

Y1 - 2020

N2 - Background: The existing modified carbapenem inactivation methods (mCIMs) recommended by the CLSI for detecting carbapenemase production have not been applicable for Acinetobacter baumannii. We evaluated the influence of matrices used in mCIMs and CIMTris on the stability of the disks for detecting carbapenemase producers and suggested optimal mCIM conditions for detecting carbapenemase-producing A. baumannii. Methods: Seventy-three A. baumannii isolates characterized for antimicrobial susceptibility and carbapenemase encoding genes were tested for carbapenemase production using mCIM and CIMTris. The influence of the matrices (Tryptic soy broth [TSB] and Tris-HCl) used in these methods on the stability of the meropenem (MEM) disk was also evaluated. The mCIM conditions were adjusted to enhance screening sensitivity and specificity for detecting carbapenemase-producing A. baumannii. Results: The matrices had an impact on the stability of the MEM disk after the incubation period (two or four hrs). TSB nutrient broth is an appropriate matrix for mCIM compared with Tris-HCl pH 7.6, which leads to the loss of MEM activity in CIMTris. The sensitivity and the specificity of the optimal mCIM were both 100%. Conclusions: We established optimal mCIM conditions for simple, accurate, and reproducible detection of carbapenemase-producing A. baumannii.

AB - Background: The existing modified carbapenem inactivation methods (mCIMs) recommended by the CLSI for detecting carbapenemase production have not been applicable for Acinetobacter baumannii. We evaluated the influence of matrices used in mCIMs and CIMTris on the stability of the disks for detecting carbapenemase producers and suggested optimal mCIM conditions for detecting carbapenemase-producing A. baumannii. Methods: Seventy-three A. baumannii isolates characterized for antimicrobial susceptibility and carbapenemase encoding genes were tested for carbapenemase production using mCIM and CIMTris. The influence of the matrices (Tryptic soy broth [TSB] and Tris-HCl) used in these methods on the stability of the meropenem (MEM) disk was also evaluated. The mCIM conditions were adjusted to enhance screening sensitivity and specificity for detecting carbapenemase-producing A. baumannii. Results: The matrices had an impact on the stability of the MEM disk after the incubation period (two or four hrs). TSB nutrient broth is an appropriate matrix for mCIM compared with Tris-HCl pH 7.6, which leads to the loss of MEM activity in CIMTris. The sensitivity and the specificity of the optimal mCIM were both 100%. Conclusions: We established optimal mCIM conditions for simple, accurate, and reproducible detection of carbapenemase-producing A. baumannii.

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AN - SCOPUS:85071774182

SN - 2234-3806

VL - 40

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JO - Annals of laboratory medicine

JF - Annals of laboratory medicine

IS - 1

ER -

Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing Acinetobacter baumannii

Abstract

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