Adjunctive biomarkers for improving diagnosis of tuberculosis and monitoring therapeutic effects

Yun Gyoung Hur; Young Ae Kang; Sun Hee Jang; Ji Young Hong; Ahreum Kim; Sang A. Lee; Youngmi Kim; Sang Nae Cho

doi:10.1016/j.jinf.2014.10.019

Adjunctive biomarkers for improving diagnosis of tuberculosis and monitoring therapeutic effects

Yun Gyoung Hur, Young Ae Kang, Sun Hee Jang, Ji Young Hong, Ahreum Kim, Sang A. Lee, Youngmi Kim, Sang Nae Cho

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Objectives: To identify host biomarkers associated with latent tuberculosis infection (LTBI), active tuberculosis (TB), and nontuberculous mycobacteria (NTM) diseases to improve diagnosis and effective anti-TB treatment. Methods: Active TB and NTM patients at diagnosis, recent TB contacts, and normal healthy subjects were recruited. Tuberculin skin tests, QuantiFERON-TB Gold In-Tube tests, and multiplex bead arrays with 17 analytes were performed. TB patients were re-evaluated after 2 and 6 months of treatment. Results: Mycobacterium tuberculosis (M. tb) antigen-specific IFN-γ, IL-2, and CXCL10 responses were significantly higher in active TB and LTBI compared with controls (P<0.01). Only serum VEGF levels varied between the active TB and LTBI groups (AUC=0.7576, P<0.001). Active TB and NTM diseases were differentiated by serum IL-2, IL-9, IL-13, IL-17, TNF-α and sCD40L levels (P<0.05). Increased sCD40L and decreased M. tb antigen-specific IFN-γ levels correlated with sputum clearance of M. tb after 2 months of treatment (P<0.001). Conclusions: Serum IL-2, IL-9, IL-13, IL-17, TNF-α, sCD40L and VEGF-A levels may be adjunctive biomarkers for differential diagnosis of active TB, LTBI, and NTM disease. Assessment of serum sCD40L and M. tb antigen-specific IFN-γ, TNF-α, and IL-2 levels could help predict successful anti-TB treatment in conjunction with M. tb clearance.

Original language	English
Pages (from-to)	346-355
Number of pages	10
Journal	Journal of Infection
Volume	70
Issue number	4
DOIs	https://doi.org/10.1016/j.jinf.2014.10.019
Publication status	Published - 2015 Apr 1

Bibliographical note

Funding Information:
We thank the study participants who contributed to this work and we appreciate the staff at Severance Hospital in Seoul, South Korea for their assistance. This study was financially supported by the Ministry for Health, Welfare, and Family Affairs, Republic of Korea (Korean Health Technology R&D Project: A101750 ) and the National Research Foundation of Korea ( 2011-0013018 ). The funding sources had no role in the study process including the design, sample collection, analysis, and interpretation of the results.

Publisher Copyright:
© 2014 The Authors.

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.jinf.2014.10.019

Cite this

@article{3386b1af587048aa858f4ac370592054,

title = "Adjunctive biomarkers for improving diagnosis of tuberculosis and monitoring therapeutic effects",

abstract = "Objectives: To identify host biomarkers associated with latent tuberculosis infection (LTBI), active tuberculosis (TB), and nontuberculous mycobacteria (NTM) diseases to improve diagnosis and effective anti-TB treatment. Methods: Active TB and NTM patients at diagnosis, recent TB contacts, and normal healthy subjects were recruited. Tuberculin skin tests, QuantiFERON-TB Gold In-Tube tests, and multiplex bead arrays with 17 analytes were performed. TB patients were re-evaluated after 2 and 6 months of treatment. Results: Mycobacterium tuberculosis (M. tb) antigen-specific IFN-γ, IL-2, and CXCL10 responses were significantly higher in active TB and LTBI compared with controls (P<0.01). Only serum VEGF levels varied between the active TB and LTBI groups (AUC=0.7576, P<0.001). Active TB and NTM diseases were differentiated by serum IL-2, IL-9, IL-13, IL-17, TNF-α and sCD40L levels (P<0.05). Increased sCD40L and decreased M. tb antigen-specific IFN-γ levels correlated with sputum clearance of M. tb after 2 months of treatment (P<0.001). Conclusions: Serum IL-2, IL-9, IL-13, IL-17, TNF-α, sCD40L and VEGF-A levels may be adjunctive biomarkers for differential diagnosis of active TB, LTBI, and NTM disease. Assessment of serum sCD40L and M. tb antigen-specific IFN-γ, TNF-α, and IL-2 levels could help predict successful anti-TB treatment in conjunction with M. tb clearance.",

author = "Hur, {Yun Gyoung} and Kang, {Young Ae} and Jang, {Sun Hee} and Hong, {Ji Young} and Ahreum Kim and Lee, {Sang A.} and Youngmi Kim and Cho, {Sang Nae}",

note = "Funding Information: We thank the study participants who contributed to this work and we appreciate the staff at Severance Hospital in Seoul, South Korea for their assistance. This study was financially supported by the Ministry for Health, Welfare, and Family Affairs, Republic of Korea (Korean Health Technology R&D Project: A101750 ) and the National Research Foundation of Korea ( 2011-0013018 ). The funding sources had no role in the study process including the design, sample collection, analysis, and interpretation of the results. Publisher Copyright: {\textcopyright} 2014 The Authors.",

year = "2015",

month = apr,

day = "1",

doi = "10.1016/j.jinf.2014.10.019",

language = "English",

volume = "70",

pages = "346--355",

journal = "Journal of Infection",

issn = "0163-4453",

publisher = "W.B. Saunders Ltd",

number = "4",

}

TY - JOUR

T1 - Adjunctive biomarkers for improving diagnosis of tuberculosis and monitoring therapeutic effects

AU - Hur, Yun Gyoung

AU - Kang, Young Ae

AU - Jang, Sun Hee

AU - Hong, Ji Young

AU - Kim, Ahreum

AU - Lee, Sang A.

AU - Kim, Youngmi

AU - Cho, Sang Nae

N1 - Funding Information: We thank the study participants who contributed to this work and we appreciate the staff at Severance Hospital in Seoul, South Korea for their assistance. This study was financially supported by the Ministry for Health, Welfare, and Family Affairs, Republic of Korea (Korean Health Technology R&D Project: A101750 ) and the National Research Foundation of Korea ( 2011-0013018 ). The funding sources had no role in the study process including the design, sample collection, analysis, and interpretation of the results. Publisher Copyright: © 2014 The Authors.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Objectives: To identify host biomarkers associated with latent tuberculosis infection (LTBI), active tuberculosis (TB), and nontuberculous mycobacteria (NTM) diseases to improve diagnosis and effective anti-TB treatment. Methods: Active TB and NTM patients at diagnosis, recent TB contacts, and normal healthy subjects were recruited. Tuberculin skin tests, QuantiFERON-TB Gold In-Tube tests, and multiplex bead arrays with 17 analytes were performed. TB patients were re-evaluated after 2 and 6 months of treatment. Results: Mycobacterium tuberculosis (M. tb) antigen-specific IFN-γ, IL-2, and CXCL10 responses were significantly higher in active TB and LTBI compared with controls (P<0.01). Only serum VEGF levels varied between the active TB and LTBI groups (AUC=0.7576, P<0.001). Active TB and NTM diseases were differentiated by serum IL-2, IL-9, IL-13, IL-17, TNF-α and sCD40L levels (P<0.05). Increased sCD40L and decreased M. tb antigen-specific IFN-γ levels correlated with sputum clearance of M. tb after 2 months of treatment (P<0.001). Conclusions: Serum IL-2, IL-9, IL-13, IL-17, TNF-α, sCD40L and VEGF-A levels may be adjunctive biomarkers for differential diagnosis of active TB, LTBI, and NTM disease. Assessment of serum sCD40L and M. tb antigen-specific IFN-γ, TNF-α, and IL-2 levels could help predict successful anti-TB treatment in conjunction with M. tb clearance.

AB - Objectives: To identify host biomarkers associated with latent tuberculosis infection (LTBI), active tuberculosis (TB), and nontuberculous mycobacteria (NTM) diseases to improve diagnosis and effective anti-TB treatment. Methods: Active TB and NTM patients at diagnosis, recent TB contacts, and normal healthy subjects were recruited. Tuberculin skin tests, QuantiFERON-TB Gold In-Tube tests, and multiplex bead arrays with 17 analytes were performed. TB patients were re-evaluated after 2 and 6 months of treatment. Results: Mycobacterium tuberculosis (M. tb) antigen-specific IFN-γ, IL-2, and CXCL10 responses were significantly higher in active TB and LTBI compared with controls (P<0.01). Only serum VEGF levels varied between the active TB and LTBI groups (AUC=0.7576, P<0.001). Active TB and NTM diseases were differentiated by serum IL-2, IL-9, IL-13, IL-17, TNF-α and sCD40L levels (P<0.05). Increased sCD40L and decreased M. tb antigen-specific IFN-γ levels correlated with sputum clearance of M. tb after 2 months of treatment (P<0.001). Conclusions: Serum IL-2, IL-9, IL-13, IL-17, TNF-α, sCD40L and VEGF-A levels may be adjunctive biomarkers for differential diagnosis of active TB, LTBI, and NTM disease. Assessment of serum sCD40L and M. tb antigen-specific IFN-γ, TNF-α, and IL-2 levels could help predict successful anti-TB treatment in conjunction with M. tb clearance.

UR - http://www.scopus.com/inward/record.url?scp=84926115962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926115962&partnerID=8YFLogxK

U2 - 10.1016/j.jinf.2014.10.019

DO - 10.1016/j.jinf.2014.10.019

M3 - Article

C2 - 25452040

AN - SCOPUS:84926115962

SN - 0163-4453

VL - 70

SP - 346

EP - 355

JO - Journal of Infection

JF - Journal of Infection

IS - 4

ER -

Adjunctive biomarkers for improving diagnosis of tuberculosis and monitoring therapeutic effects

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this