TY - JOUR
T1 - Acidic polysaccharide from the edible insect Protaetia brevitarsis seulensis activates antiviral immunity to suppress norovirus infection
AU - Olawuyi, Ibukunoluwa Fola
AU - Heo, Eun
AU - Jeong, Minju
AU - Kim, Jae Hwan
AU - Park, Jong Jin
AU - Chae, Jongbeom
AU - Gwon, Subin
AU - Do Lee, Seong
AU - Kim, Hunseong
AU - Ojulari, Oyindamola Vivian
AU - Song, Young Bo
AU - Lee, Byung Hoo
AU - Gu, Bon Bin
AU - Kim, Soo Rin
AU - Lee, Joon Ha
AU - Lee, Wonyoung
AU - Hwang, Jae Sam
AU - Nam, Ju Ock
AU - Hahn, Dongyup
AU - Byun, Sanguine
N1 - Publisher Copyright:
© 2024 Elsevier Ltd
PY - 2025/1/1
Y1 - 2025/1/1
N2 - Edible insects are gaining attention as potential nutraceutical sources with immunomodulatory properties. This study reports purification and structural characterization of polysaccharides from Protaetia brevitarsis seulensis larvae (PBSL) with antiviral activity against murine norovirus. Four polysaccharide fractions purified from PBSL water extracts exhibited varying molecular weights (458.5–627.3 kDa) and monosaccharide compositions, including glucose (42.4–99.2 %), galactose (5.9–13.9 %), rhamnose (0.7–18.7 %), arabinose (3.8–5.4 %), and glucuronic acid (0–15.3 %). The immunomodulatory activity, assessed by interferon-β (IFN-β) production, positively correlated with higher galactose, mannose, rhamnose, and uronic acid contents. Among the fractions, PBS-P, eluted with 0.5 M NaCl, demonstrated superior in vitro antiviral activity with IFN-β production exceeding 8-fold compared to other fractions and 82-fold higher than PBSL water extract, confirming it as the main antiviral active component. Structural analysis revealed PBS-P backbone consisted of α-(1 → 4)-D-Glcp, α-(1 → 4,6)-D-Glcp, α-(1 → 4)-D-GlcpA, α-(1 → 3)-D-Galp and α-(1 → 4)-D-Manp residues, and branched chains of α-D-Glcp-(1→, and α-L-Arap-(1 → 2)-α-L-Rhap-(1 → residues. PBS-P suppressed norovirus replication by stimulating IFN-β, TNF-α, and activating NF-κB, STAT1/2, and TBK1-IRF3 pathways, and its oral administration reduced viral loads in infected mice intestines. This study provides the first report on the detailed structural feature of polysaccharide from an edible insect and its antiviral mechanism, highlighting its potential as a new antiviral agent.
AB - Edible insects are gaining attention as potential nutraceutical sources with immunomodulatory properties. This study reports purification and structural characterization of polysaccharides from Protaetia brevitarsis seulensis larvae (PBSL) with antiviral activity against murine norovirus. Four polysaccharide fractions purified from PBSL water extracts exhibited varying molecular weights (458.5–627.3 kDa) and monosaccharide compositions, including glucose (42.4–99.2 %), galactose (5.9–13.9 %), rhamnose (0.7–18.7 %), arabinose (3.8–5.4 %), and glucuronic acid (0–15.3 %). The immunomodulatory activity, assessed by interferon-β (IFN-β) production, positively correlated with higher galactose, mannose, rhamnose, and uronic acid contents. Among the fractions, PBS-P, eluted with 0.5 M NaCl, demonstrated superior in vitro antiviral activity with IFN-β production exceeding 8-fold compared to other fractions and 82-fold higher than PBSL water extract, confirming it as the main antiviral active component. Structural analysis revealed PBS-P backbone consisted of α-(1 → 4)-D-Glcp, α-(1 → 4,6)-D-Glcp, α-(1 → 4)-D-GlcpA, α-(1 → 3)-D-Galp and α-(1 → 4)-D-Manp residues, and branched chains of α-D-Glcp-(1→, and α-L-Arap-(1 → 2)-α-L-Rhap-(1 → residues. PBS-P suppressed norovirus replication by stimulating IFN-β, TNF-α, and activating NF-κB, STAT1/2, and TBK1-IRF3 pathways, and its oral administration reduced viral loads in infected mice intestines. This study provides the first report on the detailed structural feature of polysaccharide from an edible insect and its antiviral mechanism, highlighting its potential as a new antiviral agent.
KW - Immunomodulation
KW - Insect larvae
KW - Norovirus
KW - Polysaccharides
KW - Structure
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UR - http://www.scopus.com/inward/citedby.url?scp=85204449727&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2024.122587
DO - 10.1016/j.carbpol.2024.122587
M3 - Article
C2 - 39486915
AN - SCOPUS:85204449727
SN - 0144-8617
VL - 347
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 122587
ER -