A study of compatibility between cells and biopolymeric surfaces through quantitative measurements of adhesive forces

Young Jick Kim, Jung Woog Shin, Ki Dong Park, Jin Woo Lee, Nobuhiko Yui, Su A. Park, Kyoung Soo Jee, Jeong Koo Kim

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


The mechanism of cell adhesion to biomaterials or components of the extracellular matrix is an important topic in the field of tissue engineering and related biotechnological processes. Many factors affect cell adhesion, and many biochemical and biological studies have attempted to identify their roles in the adhesion mechanism. Systematic studies of this nature require quantification of the adhesive force of a cell to identify the effect of a specific factor. However, most studies of cell adhesive force have used qualitative approaches. We propose a new technique for quantifying the force by which cells adhere to various biomaterial surfaces, which utilizes the relationship between the deflection of a cantilever beam and the required force. A micropipette was used as the cantilever beam. This technique was used to measure the attachment forces of chondrocytes seeded on three different biodegradable polymers commonly used in tissue engineering and medicine: poly E-carprolactone (PCL), poly(L-lactide) (PLLA) and poly(lactic-co-glycolic acid) (PGLA, L/G = 75: 25). The bond between the cells and the three polymers was evaluated using the quantified adhesive forces. The adhesive forces were also measured 8, 12, 24 h and 5 days after seeding the chondrocytes on the polymer surfaces. Results of statistical analysis showed that the cells attached to the PLLA had the strongest average attachment force for up to 24 h after seeding (P < 0.05).

Original languageEnglish
Pages (from-to)1311-1321
Number of pages11
JournalJournal of Biomaterials Science, Polymer Edition
Issue number12
Publication statusPublished - 2003

Bibliographical note

Funding Information:
This work was supported by the Korea Sceceinand Engineering Foundona(Projectti No. 2000-2-31400-005-3).

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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