A single gene of a commensal microbe affects host susceptibility to enteric infection

Mi Young Yoon; Kyung Bae Min; Kang Mu Lee; Yujin Yoon; Yaeseul Kim; Young Taek Oh; Keehoon Lee; Jongsik Chun; Byung Yong Kim; Seok Hwan Yoon; Insuk Lee; Chan Yeong Kim; Sang Sun Yoon

doi:10.1038/ncomms11606

A single gene of a commensal microbe affects host susceptibility to enteric infection

Mi Young Yoon, Kyung Bae Min, Kang Mu Lee, Yujin Yoon, Yaeseul Kim, Young Taek Oh, Keehoon Lee, Jongsik Chun, Byung Yong Kim, Seok Hwan Yoon, Insuk Lee, Chan Yeong Kim, Sang Sun Yoon

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Indigenous microbes inside the host intestine maintain a complex self-regulating community. The mechanisms by which gut microbes interact with intestinal pathogens remain largely unknown. Here we identify a commensal Escherichia coli strain whose expansion predisposes mice to infection by Vibrio cholerae, a human pathogen. We refer to this strain as 'atypical' E. coli (atEc) because of its inability to ferment lactose. The atEc strain is resistant to reactive oxygen species (ROS) and proliferates extensively in antibiotic-treated adult mice. V. cholerae infection is more severe in neonatal mice transplanted with atEc compared with those transplanted with a typical E. coli strain. Intestinal ROS levels are decreased in atEc-transplanted mice, favouring proliferation of ROS-sensitive V. cholerae. An atEc mutant defective in ROS degradation fails to facilitate V. cholerae infection when transplanted, suggesting that host infection susceptibility can be regulated by a single gene product of one particular commensal species.

Original language	English
Article number	11606
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms11606
Publication status	Published - 2016 May 13

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation (NRF) of Korea, funded by the Korean Government (MSIP), 2014R1A2A2A01002861, 2014R1A4A1008625, 2015M3C9A2054020 and 2014R1A1A1006403. This work was also supported by a grant from the Korean Ministry of Health & Welfare, HI14C1324. We appreciate the assistance of Dr. Byung Kwon Kim (OmicsPia, Inc., Taejun, Korea) for bioinformatics analyses.

Publisher Copyright:
© 2016, Nature Publishing Group. All rights reserved.

All Science Journal Classification (ASJC) codes

General
Physics and Astronomy(all)
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)

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title = "A single gene of a commensal microbe affects host susceptibility to enteric infection",

abstract = "Indigenous microbes inside the host intestine maintain a complex self-regulating community. The mechanisms by which gut microbes interact with intestinal pathogens remain largely unknown. Here we identify a commensal Escherichia coli strain whose expansion predisposes mice to infection by Vibrio cholerae, a human pathogen. We refer to this strain as 'atypical' E. coli (atEc) because of its inability to ferment lactose. The atEc strain is resistant to reactive oxygen species (ROS) and proliferates extensively in antibiotic-treated adult mice. V. cholerae infection is more severe in neonatal mice transplanted with atEc compared with those transplanted with a typical E. coli strain. Intestinal ROS levels are decreased in atEc-transplanted mice, favouring proliferation of ROS-sensitive V. cholerae. An atEc mutant defective in ROS degradation fails to facilitate V. cholerae infection when transplanted, suggesting that host infection susceptibility can be regulated by a single gene product of one particular commensal species.",

author = "Yoon, {Mi Young} and Min, {Kyung Bae} and Lee, {Kang Mu} and Yujin Yoon and Yaeseul Kim and Oh, {Young Taek} and Keehoon Lee and Jongsik Chun and Kim, {Byung Yong} and Yoon, {Seok Hwan} and Insuk Lee and Kim, {Chan Yeong} and Yoon, {Sang Sun}",

note = "Funding Information: This work was supported by grants from the National Research Foundation (NRF) of Korea, funded by the Korean Government (MSIP), 2014R1A2A2A01002861, 2014R1A4A1008625, 2015M3C9A2054020 and 2014R1A1A1006403. This work was also supported by a grant from the Korean Ministry of Health & Welfare, HI14C1324. We appreciate the assistance of Dr. Byung Kwon Kim (OmicsPia, Inc., Taejun, Korea) for bioinformatics analyses. Publisher Copyright: {\textcopyright} 2016, Nature Publishing Group. All rights reserved.",

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AU - Kim, Chan Yeong

AU - Yoon, Sang Sun

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A single gene of a commensal microbe affects host susceptibility to enteric infection

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