A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy

Kyujung Kim; Donghyun Kim; Eun Jin Cho; Yong Min Huh

doi:10.1117/12.699874

A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy

Kyujung Kim, Donghyun Kim, Eun Jin Cho, Yong Min Huh

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Citations (Scopus)

Abstract

For investigatoin of intracellular protein interactions, quantum dots are widely used for fluorescent live cell imaging such as total internal reflection fluorescence (TIRF) microscopy and confocal microscopy. In this paper, we performed a quantitative analysis based on fluorescent intensity. For the measurement, A431 cell lines are imaged live with quantum dots using TIRF microscopy. The distribution of quantum dots is affected by a TATHA2 peptide sequence in live cells. This paper also presents the location change of quantum dots due to a nuclear localization signal in A431 cell lines. Confocal microscopy was used to confirm the relation with fluorescent intensity and quantum dot concentration in live cells.

Original language	English
Title of host publication	Genetically Engineered and Optical Probes for Biomedical Applications IV
DOIs	https://doi.org/10.1117/12.699874
Publication status	Published - 2007
Event	Genetically Engineered and Optical Probes for Biomedical Applications IV - San Jose, CA, United States Duration: 2007 Jan 23 → 2007 Jan 24

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6449
ISSN (Print)	1605-7422

Other

Other	Genetically Engineered and Optical Probes for Biomedical Applications IV
Country/Territory	United States
City	San Jose, CA
Period	07/1/23 → 07/1/24

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.699874

Cite this

Kim, K., Kim, D., Cho, E. J., & Huh, Y. M. (2007). A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy. In Genetically Engineered and Optical Probes for Biomedical Applications IV Article 64490K (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6449). https://doi.org/10.1117/12.699874

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title = "A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy",

abstract = "For investigatoin of intracellular protein interactions, quantum dots are widely used for fluorescent live cell imaging such as total internal reflection fluorescence (TIRF) microscopy and confocal microscopy. In this paper, we performed a quantitative analysis based on fluorescent intensity. For the measurement, A431 cell lines are imaged live with quantum dots using TIRF microscopy. The distribution of quantum dots is affected by a TATHA2 peptide sequence in live cells. This paper also presents the location change of quantum dots due to a nuclear localization signal in A431 cell lines. Confocal microscopy was used to confirm the relation with fluorescent intensity and quantum dot concentration in live cells.",

author = "Kyujung Kim and Donghyun Kim and Cho, {Eun Jin} and Huh, {Yong Min}",

year = "2007",

doi = "10.1117/12.699874",

language = "English",

isbn = "0819465623",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Genetically Engineered and Optical Probes for Biomedical Applications IV",

note = "Genetically Engineered and Optical Probes for Biomedical Applications IV ; Conference date: 23-01-2007 Through 24-01-2007",

}

Kim, K, Kim, D, Cho, EJ & Huh, YM 2007, A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy. in Genetically Engineered and Optical Probes for Biomedical Applications IV., 64490K, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, San Jose, CA, United States, 07/1/23. https://doi.org/10.1117/12.699874

A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy. / Kim, Kyujung; Kim, Donghyun; Cho, Eun Jin et al.
Genetically Engineered and Optical Probes for Biomedical Applications IV. 2007. 64490K (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6449).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy

AU - Kim, Kyujung

AU - Kim, Donghyun

AU - Cho, Eun Jin

AU - Huh, Yong Min

PY - 2007

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N2 - For investigatoin of intracellular protein interactions, quantum dots are widely used for fluorescent live cell imaging such as total internal reflection fluorescence (TIRF) microscopy and confocal microscopy. In this paper, we performed a quantitative analysis based on fluorescent intensity. For the measurement, A431 cell lines are imaged live with quantum dots using TIRF microscopy. The distribution of quantum dots is affected by a TATHA2 peptide sequence in live cells. This paper also presents the location change of quantum dots due to a nuclear localization signal in A431 cell lines. Confocal microscopy was used to confirm the relation with fluorescent intensity and quantum dot concentration in live cells.

AB - For investigatoin of intracellular protein interactions, quantum dots are widely used for fluorescent live cell imaging such as total internal reflection fluorescence (TIRF) microscopy and confocal microscopy. In this paper, we performed a quantitative analysis based on fluorescent intensity. For the measurement, A431 cell lines are imaged live with quantum dots using TIRF microscopy. The distribution of quantum dots is affected by a TATHA2 peptide sequence in live cells. This paper also presents the location change of quantum dots due to a nuclear localization signal in A431 cell lines. Confocal microscopy was used to confirm the relation with fluorescent intensity and quantum dot concentration in live cells.

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M3 - Conference contribution

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SN - 0819465623

SN - 9780819465627

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Genetically Engineered and Optical Probes for Biomedical Applications IV

T2 - Genetically Engineered and Optical Probes for Biomedical Applications IV

Y2 - 23 January 2007 through 24 January 2007

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A quantitative analysis of the intracellular transport of quantum dot-peptide in live cells using total internal reflection and confocal microscopy

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