A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings

Hyung Gon Mang; Eun Ok Kang; Jung Hye Shim; So Yeon Kim; Ky Young Park; Yu Sam Kim; Young Yil Bahk; Woo Taek Kim

doi:10.1016/j.bbaexp.2003.12.005

A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings

Hyung Gon Mang, Eun Ok Kang, Jung Hye Shim, So Yeon Kim, Ky Young Park, Yu Sam Kim, Young Yil Bahk, Woo Taek Kim

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

The plant hormone ethylene has been shown to play an important role in root hair development in Arabidopsis. With the aid of proteomic analysis, we identified three distinct glutathione S-transferase (GST) isoforms, AtGSTF2, AtGSTF8, and AtGSTU19, expressed early in root epidermal establishment in Arabidopsis seedlings. The AtGSTF2 protein was specifically up-regulated by ethylene. A subsequent RNA expression study revealed that the AtGSTF2 gene was highly sensitive to ethylene, whereas the transcripts for AtGSTF8 and AtGSTU19 were constitutively present in new root tissue of 4-day-old seedlings. The steady-state level of AtGSTF2 mRNA was greatly reduced in the roots of ethylene-insensitive mutants, while mutation at the CTR1 locus, which confers an ectopic root hair phenotype, resulted in a markedly elevated level of AtGSTF2 transcript in young root tissue. Although the physiological function of ethylene-induced AtGSTF2 is not yet clear, there are several possibilities for its role during early root development.

Original language	English
Pages (from-to)	231-239
Number of pages	9
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1676
Issue number	3
DOIs	https://doi.org/10.1016/j.bbaexp.2003.12.005
Publication status	Published - 2004 Feb 20

Bibliographical note

Funding Information:
This work was supported by grants from Plant Metabolism Research Center at Kyung Hee University (Science Research Center Program from Korea Science and Engineering Foundation), Plant Diversity Research Center (21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government, project number PF0330404-00), and KRIBB (joint research program) to W.T.K., and from KOSEF (Protein Network Research Center at Yonsei University) to Y.Y.B.

All Science Journal Classification (ASJC) codes

Structural Biology
Biophysics
Biochemistry
Genetics

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Mang, H. G., Kang, E. O., Shim, J. H., Kim, S. Y., Park, K. Y., Kim, Y. S., Bahk, Y. Y., & Kim, W. T. (2004). A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings. Biochimica et Biophysica Acta - Gene Structure and Expression, 1676(3), 231-239. https://doi.org/10.1016/j.bbaexp.2003.12.005

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title = "A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings",

abstract = "The plant hormone ethylene has been shown to play an important role in root hair development in Arabidopsis. With the aid of proteomic analysis, we identified three distinct glutathione S-transferase (GST) isoforms, AtGSTF2, AtGSTF8, and AtGSTU19, expressed early in root epidermal establishment in Arabidopsis seedlings. The AtGSTF2 protein was specifically up-regulated by ethylene. A subsequent RNA expression study revealed that the AtGSTF2 gene was highly sensitive to ethylene, whereas the transcripts for AtGSTF8 and AtGSTU19 were constitutively present in new root tissue of 4-day-old seedlings. The steady-state level of AtGSTF2 mRNA was greatly reduced in the roots of ethylene-insensitive mutants, while mutation at the CTR1 locus, which confers an ectopic root hair phenotype, resulted in a markedly elevated level of AtGSTF2 transcript in young root tissue. Although the physiological function of ethylene-induced AtGSTF2 is not yet clear, there are several possibilities for its role during early root development.",

author = "Mang, {Hyung Gon} and Kang, {Eun Ok} and Shim, {Jung Hye} and Kim, {So Yeon} and Park, {Ky Young} and Kim, {Yu Sam} and Bahk, {Young Yil} and Kim, {Woo Taek}",

note = "Funding Information: This work was supported by grants from Plant Metabolism Research Center at Kyung Hee University (Science Research Center Program from Korea Science and Engineering Foundation), Plant Diversity Research Center (21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government, project number PF0330404-00), and KRIBB (joint research program) to W.T.K., and from KOSEF (Protein Network Research Center at Yonsei University) to Y.Y.B.",

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Mang, HG, Kang, EO, Shim, JH, Kim, SY, Park, KY, Kim, YS, Bahk, YY & Kim, WT 2004, 'A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings', Biochimica et Biophysica Acta - Gene Structure and Expression, vol. 1676, no. 3, pp. 231-239. https://doi.org/10.1016/j.bbaexp.2003.12.005

A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings. / Mang, Hyung Gon; Kang, Eun Ok; Shim, Jung Hye et al.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1676, No. 3, 20.02.2004, p. 231-239.

Research output: Contribution to journal › Article › peer-review

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T1 - A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings

AU - Mang, Hyung Gon

AU - Kang, Eun Ok

AU - Shim, Jung Hye

AU - Kim, So Yeon

AU - Park, Ky Young

AU - Kim, Yu Sam

AU - Bahk, Young Yil

AU - Kim, Woo Taek

N1 - Funding Information: This work was supported by grants from Plant Metabolism Research Center at Kyung Hee University (Science Research Center Program from Korea Science and Engineering Foundation), Plant Diversity Research Center (21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government, project number PF0330404-00), and KRIBB (joint research program) to W.T.K., and from KOSEF (Protein Network Research Center at Yonsei University) to Y.Y.B.

PY - 2004/2/20

Y1 - 2004/2/20

N2 - The plant hormone ethylene has been shown to play an important role in root hair development in Arabidopsis. With the aid of proteomic analysis, we identified three distinct glutathione S-transferase (GST) isoforms, AtGSTF2, AtGSTF8, and AtGSTU19, expressed early in root epidermal establishment in Arabidopsis seedlings. The AtGSTF2 protein was specifically up-regulated by ethylene. A subsequent RNA expression study revealed that the AtGSTF2 gene was highly sensitive to ethylene, whereas the transcripts for AtGSTF8 and AtGSTU19 were constitutively present in new root tissue of 4-day-old seedlings. The steady-state level of AtGSTF2 mRNA was greatly reduced in the roots of ethylene-insensitive mutants, while mutation at the CTR1 locus, which confers an ectopic root hair phenotype, resulted in a markedly elevated level of AtGSTF2 transcript in young root tissue. Although the physiological function of ethylene-induced AtGSTF2 is not yet clear, there are several possibilities for its role during early root development.

AB - The plant hormone ethylene has been shown to play an important role in root hair development in Arabidopsis. With the aid of proteomic analysis, we identified three distinct glutathione S-transferase (GST) isoforms, AtGSTF2, AtGSTF8, and AtGSTU19, expressed early in root epidermal establishment in Arabidopsis seedlings. The AtGSTF2 protein was specifically up-regulated by ethylene. A subsequent RNA expression study revealed that the AtGSTF2 gene was highly sensitive to ethylene, whereas the transcripts for AtGSTF8 and AtGSTU19 were constitutively present in new root tissue of 4-day-old seedlings. The steady-state level of AtGSTF2 mRNA was greatly reduced in the roots of ethylene-insensitive mutants, while mutation at the CTR1 locus, which confers an ectopic root hair phenotype, resulted in a markedly elevated level of AtGSTF2 transcript in young root tissue. Although the physiological function of ethylene-induced AtGSTF2 is not yet clear, there are several possibilities for its role during early root development.

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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Mang HG, Kang EO, Shim JH, Kim SY, Park KY, Kim YS et al. A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings. Biochimica et Biophysica Acta - Gene Structure and Expression. 2004 Feb 20;1676(3):231-239. doi: 10.1016/j.bbaexp.2003.12.005

A proteomic analysis identifies glutathione S-transferase isoforms whose abundance is differentially regulated by ethylene during the formation of early root epidermis in Arabidopsis seedlings

Abstract

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