A novel microneedle device for controlled and reliable liquid biopsy of the human inner ear

Samuel Early; In Seok Moon; Krishna Bommakanti; Ian Hunter; Konstantina M. Stankovic

doi:10.1016/j.heares.2019.06.004

A novel microneedle device for controlled and reliable liquid biopsy of the human inner ear

Samuel Early, In Seok Moon, Krishna Bommakanti, Ian Hunter, Konstantina M. Stankovic

Department of Otorhinolaryngology

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Sensorineural hearing loss is the most common sensory deficit worldwide, yet our understanding of the underlying pathophysiology is limited by the challenges of access to the inner ear in a safe and reliable manner. We present a novel microneedle device for trans-round window membrane liquid biopsy, which utilizes controlled depth of perforation and microliter aspiration control to safely biopsy fluids of the inner ear. Of eleven devices tested in fresh frozen human temporal bones, seven demonstrated alignment between electrical, visual and tactile detection of round window membrane perforation, and nine were successful in aspiration of meaningful diagnostic samples from the perilymphatic space. Purity of the average perilymph sample was 69% for a 5 μL sample volume, equivalent to 3.5 μL attributable to perilymph. Diagnostic success was shown both by transmastoid facial recess and transcanal tympanotomy approach. This device can enable new advances in the understanding of inner ear pathology, and brings us one step closer to liquid biopsy of the inner ear becoming a routine part of clinical care.

Original language	English
Article number	107761
Journal	Hearing Research
Volume	381
DOIs	https://doi.org/10.1016/j.heares.2019.06.004
Publication status	Published - 2019 Sept 15

Bibliographical note

Funding Information:
This work was supported by the National Institute on Deafness and Other Communication Disorders grant R01DC015824, Nancy Sayles Day Foundation, the Lauer Tinnitus Research Center, Mona Taliaferro and Sheldon and Dorothea Buckler. We thank Jean Phillips for her support in maintaining and operating the Joseph B. Nadol, Jr. M.D. Otolaryngology Surgical Training Laboratory at Mass Eye and Ear, where all human in situ measurements for this study were performed. We thank also Diane Jones of the Mass Eye and Ear Otopathology Laboratory for her services in acquiring and storing the human temporal bones needed for this study.

Funding Information:
This work was supported by the National Institute on Deafness and Other Communication Disorders grant R01DC015824 , Nancy Sayles Day Foundation , the Lauer Tinnitus Research Center , Mona Taliaferro and Sheldon and Dorothea Buckler.

Publisher Copyright:
© 2019

All Science Journal Classification (ASJC) codes

Sensory Systems

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10.1016/j.heares.2019.06.004

Cite this

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title = "A novel microneedle device for controlled and reliable liquid biopsy of the human inner ear",

abstract = "Sensorineural hearing loss is the most common sensory deficit worldwide, yet our understanding of the underlying pathophysiology is limited by the challenges of access to the inner ear in a safe and reliable manner. We present a novel microneedle device for trans-round window membrane liquid biopsy, which utilizes controlled depth of perforation and microliter aspiration control to safely biopsy fluids of the inner ear. Of eleven devices tested in fresh frozen human temporal bones, seven demonstrated alignment between electrical, visual and tactile detection of round window membrane perforation, and nine were successful in aspiration of meaningful diagnostic samples from the perilymphatic space. Purity of the average perilymph sample was 69% for a 5 μL sample volume, equivalent to 3.5 μL attributable to perilymph. Diagnostic success was shown both by transmastoid facial recess and transcanal tympanotomy approach. This device can enable new advances in the understanding of inner ear pathology, and brings us one step closer to liquid biopsy of the inner ear becoming a routine part of clinical care.",

author = "Samuel Early and Moon, {In Seok} and Krishna Bommakanti and Ian Hunter and Stankovic, {Konstantina M.}",

note = "Funding Information: This work was supported by the National Institute on Deafness and Other Communication Disorders grant R01DC015824, Nancy Sayles Day Foundation, the Lauer Tinnitus Research Center, Mona Taliaferro and Sheldon and Dorothea Buckler. We thank Jean Phillips for her support in maintaining and operating the Joseph B. Nadol, Jr. M.D. Otolaryngology Surgical Training Laboratory at Mass Eye and Ear, where all human in situ measurements for this study were performed. We thank also Diane Jones of the Mass Eye and Ear Otopathology Laboratory for her services in acquiring and storing the human temporal bones needed for this study. Funding Information: This work was supported by the National Institute on Deafness and Other Communication Disorders grant R01DC015824 , Nancy Sayles Day Foundation , the Lauer Tinnitus Research Center , Mona Taliaferro and Sheldon and Dorothea Buckler. Publisher Copyright: {\textcopyright} 2019",

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A novel microneedle device for controlled and reliable liquid biopsy of the human inner ear

Abstract

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