A novel approach to quantitate protein expression differences in human hepatocellular carcinoma

Kang Sik Park; Hoguen Kim; Yong Ki Paik; Jason N. Marchese; Brian Williamson; Peter Juhasz; Dale Patterson; Steve Martin; Vince C. Gao

A novel approach to quantitate protein expression differences in human hepatocellular carcinoma

Kang Sik Park, Hoguen Kim, Yong Ki Paik, Jason N. Marchese, Brian Williamson, Peter Juhasz, Dale Patterson, Steve Martin, Vince C. Gao

Department of Biochemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

An industrial-scale platform based on a platform composed of a Applied Biosystems ICAT^TM reagent, MDLC purification and tandem mass spectrometry systems to quantitate differential protein expression in normal and cancerous human liver tissue was described. The normal and cancerous human liver tissue were diced from the same patient and the buffered tissues were homogenized, sonicated and ultracentrifuged. The automated MDLC system was used for the separation and collection of the digested peptides. The results show that the platform offers much higher throughput and richer information content for quantitative protein analysis in complicated biological samples.

Original language	English
Pages	317-318
Number of pages	2
Publication status	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2002 Jun 2 → 2002 Jun 6

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	02/6/2 → 02/6/6

All Science Journal Classification (ASJC) codes

Spectroscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "A novel approach to quantitate protein expression differences in human hepatocellular carcinoma",

abstract = "An industrial-scale platform based on a platform composed of a Applied Biosystems ICATTM reagent, MDLC purification and tandem mass spectrometry systems to quantitate differential protein expression in normal and cancerous human liver tissue was described. The normal and cancerous human liver tissue were diced from the same patient and the buffered tissues were homogenized, sonicated and ultracentrifuged. The automated MDLC system was used for the separation and collection of the digested peptides. The results show that the platform offers much higher throughput and richer information content for quantitative protein analysis in complicated biological samples.",

author = "Park, {Kang Sik} and Hoguen Kim and Paik, {Yong Ki} and Marchese, {Jason N.} and Brian Williamson and Peter Juhasz and Dale Patterson and Steve Martin and Gao, {Vince C.}",

year = "2002",

language = "English",

pages = "317--318",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - CONF

T1 - A novel approach to quantitate protein expression differences in human hepatocellular carcinoma

AU - Park, Kang Sik

AU - Kim, Hoguen

AU - Paik, Yong Ki

AU - Marchese, Jason N.

AU - Williamson, Brian

AU - Juhasz, Peter

AU - Patterson, Dale

AU - Martin, Steve

AU - Gao, Vince C.

PY - 2002

Y1 - 2002

N2 - An industrial-scale platform based on a platform composed of a Applied Biosystems ICATTM reagent, MDLC purification and tandem mass spectrometry systems to quantitate differential protein expression in normal and cancerous human liver tissue was described. The normal and cancerous human liver tissue were diced from the same patient and the buffered tissues were homogenized, sonicated and ultracentrifuged. The automated MDLC system was used for the separation and collection of the digested peptides. The results show that the platform offers much higher throughput and richer information content for quantitative protein analysis in complicated biological samples.

AB - An industrial-scale platform based on a platform composed of a Applied Biosystems ICATTM reagent, MDLC purification and tandem mass spectrometry systems to quantitate differential protein expression in normal and cancerous human liver tissue was described. The normal and cancerous human liver tissue were diced from the same patient and the buffered tissues were homogenized, sonicated and ultracentrifuged. The automated MDLC system was used for the separation and collection of the digested peptides. The results show that the platform offers much higher throughput and richer information content for quantitative protein analysis in complicated biological samples.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442655648&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442655648

SP - 317

EP - 318

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

A novel approach to quantitate protein expression differences in human hepatocellular carcinoma

Abstract

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All Science Journal Classification (ASJC) codes

UN SDGs

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