A microfluidic array for large-scale ordering and orientation of embryos

Kwanghun Chung; Yoosik Kim; Jitendra S. Kanodia; Emily Gong; Stanislav Y. Shvartsman; Hang Lu

doi:10.1038/nmeth.1548

A microfluidic array for large-scale ordering and orientation of embryos

Kwanghun Chung, Yoosik Kim, Jitendra S. Kanodia, Emily Gong, Stanislav Y. Shvartsman, Hang Lu

Yonsei Advanced Science Institute

Research output: Contribution to journal › Article › peer-review

126 Citations (Scopus)

Abstract

Quantitative studies of embryogenesis require the ability to monitor pattern formation and morphogenesis in large numbers of embryos, at multiple time points and in diverse genetic backgrounds. We describe a simple approach that greatly facilitates these tasks for Drosophila melanogaster embryos, one of the most advanced models of developmental genetics. Based on passive hydrodynamics, we developed a microfluidic embryo-trap array that can be used to rapidly order and vertically orient hundreds of embryos. We describe the physical principles of the design and used this platform to quantitatively analyze multiple morphogen gradients in the dorsoventral patterning system. Our approach can also be used for live imaging and, with slight modifications, could be adapted for studies of pattern formation and morphogenesis in other model organisms.

Original language	English
Pages (from-to)	171-176
Number of pages	6
Journal	Nature Methods
Volume	8
Issue number	2
DOIs	https://doi.org/10.1038/nmeth.1548
Publication status	Published - 2011 Feb

Bibliographical note

Funding Information:
We acknowledge A. Boettiger and M. Levine (University of California, Berkeley) for the antibody to Twist, M. Zhan for technical assistance, A. Boettiger, A. Erives, M. Levine, J. Lippincott-Schwartz, C. Rushlow, M. Serpe and R. Steward for helpful discussions, and M. Osterfield for assistance with live imaging. This work was supported by National Science Foundation (DBI-0649833 to H.L.) and National Institutes of Health grants NS058465 (to H.L.) and GM078079 (to S.Y.S.). H.L. is supported by a Sloan Foundation Research Fellowship and a DuPont Young Professor grant.

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/nmeth.1548

Cite this

@article{51615ff66182405495d3285406ce0794,

title = "A microfluidic array for large-scale ordering and orientation of embryos",

abstract = "Quantitative studies of embryogenesis require the ability to monitor pattern formation and morphogenesis in large numbers of embryos, at multiple time points and in diverse genetic backgrounds. We describe a simple approach that greatly facilitates these tasks for Drosophila melanogaster embryos, one of the most advanced models of developmental genetics. Based on passive hydrodynamics, we developed a microfluidic embryo-trap array that can be used to rapidly order and vertically orient hundreds of embryos. We describe the physical principles of the design and used this platform to quantitatively analyze multiple morphogen gradients in the dorsoventral patterning system. Our approach can also be used for live imaging and, with slight modifications, could be adapted for studies of pattern formation and morphogenesis in other model organisms.",

author = "Kwanghun Chung and Yoosik Kim and Kanodia, {Jitendra S.} and Emily Gong and Shvartsman, {Stanislav Y.} and Hang Lu",

note = "Funding Information: We acknowledge A. Boettiger and M. Levine (University of California, Berkeley) for the antibody to Twist, M. Zhan for technical assistance, A. Boettiger, A. Erives, M. Levine, J. Lippincott-Schwartz, C. Rushlow, M. Serpe and R. Steward for helpful discussions, and M. Osterfield for assistance with live imaging. This work was supported by National Science Foundation (DBI-0649833 to H.L.) and National Institutes of Health grants NS058465 (to H.L.) and GM078079 (to S.Y.S.). H.L. is supported by a Sloan Foundation Research Fellowship and a DuPont Young Professor grant.",

year = "2011",

month = feb,

doi = "10.1038/nmeth.1548",

language = "English",

volume = "8",

pages = "171--176",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "2",

}

TY - JOUR

T1 - A microfluidic array for large-scale ordering and orientation of embryos

AU - Chung, Kwanghun

AU - Kim, Yoosik

AU - Kanodia, Jitendra S.

AU - Gong, Emily

AU - Shvartsman, Stanislav Y.

AU - Lu, Hang

N1 - Funding Information: We acknowledge A. Boettiger and M. Levine (University of California, Berkeley) for the antibody to Twist, M. Zhan for technical assistance, A. Boettiger, A. Erives, M. Levine, J. Lippincott-Schwartz, C. Rushlow, M. Serpe and R. Steward for helpful discussions, and M. Osterfield for assistance with live imaging. This work was supported by National Science Foundation (DBI-0649833 to H.L.) and National Institutes of Health grants NS058465 (to H.L.) and GM078079 (to S.Y.S.). H.L. is supported by a Sloan Foundation Research Fellowship and a DuPont Young Professor grant.

PY - 2011/2

Y1 - 2011/2

N2 - Quantitative studies of embryogenesis require the ability to monitor pattern formation and morphogenesis in large numbers of embryos, at multiple time points and in diverse genetic backgrounds. We describe a simple approach that greatly facilitates these tasks for Drosophila melanogaster embryos, one of the most advanced models of developmental genetics. Based on passive hydrodynamics, we developed a microfluidic embryo-trap array that can be used to rapidly order and vertically orient hundreds of embryos. We describe the physical principles of the design and used this platform to quantitatively analyze multiple morphogen gradients in the dorsoventral patterning system. Our approach can also be used for live imaging and, with slight modifications, could be adapted for studies of pattern formation and morphogenesis in other model organisms.

AB - Quantitative studies of embryogenesis require the ability to monitor pattern formation and morphogenesis in large numbers of embryos, at multiple time points and in diverse genetic backgrounds. We describe a simple approach that greatly facilitates these tasks for Drosophila melanogaster embryos, one of the most advanced models of developmental genetics. Based on passive hydrodynamics, we developed a microfluidic embryo-trap array that can be used to rapidly order and vertically orient hundreds of embryos. We describe the physical principles of the design and used this platform to quantitatively analyze multiple morphogen gradients in the dorsoventral patterning system. Our approach can also be used for live imaging and, with slight modifications, could be adapted for studies of pattern formation and morphogenesis in other model organisms.

UR - http://www.scopus.com/inward/record.url?scp=79551572775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551572775&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1548

DO - 10.1038/nmeth.1548

M3 - Article

C2 - 21186361

AN - SCOPUS:79551572775

SN - 1548-7091

VL - 8

SP - 171

EP - 176

JO - Nature Methods

JF - Nature Methods

IS - 2

ER -

A microfluidic array for large-scale ordering and orientation of embryos

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this