A highly sensitive amperometric galactose biosensor was developed by encapsulating galactose oxidase within the graphene-doped sol-gel titania-Nafion composite film on platinized glassy carbon electrode. Due to the combined electrocatalytic activity of graphene and Pt NPs on the electrode towards hydrogen peroxide as well as the mesoporous nature of the titania-Nafion composite, the present galactose biosensor exhibited relatively fast response time under 2 s, high sensitivity of 40.6 mAM−1cm−2, and wide dynamic range over three orders of magnitude with a detection limit of 3.78×10−6 M (S/N=3). In addition, the biocompatible composite in the biosensor secures excellent long-term stability.
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry