A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

Howon Lee, Hyoki Kim, Sungsik Kim, Taehoon Ryu, Hwangbeom Kim, Duhee Bang, Sunghoon Kwon

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called â € Sniper Cloningâ €™ that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.

Original languageEnglish
Article number6073
JournalNature communications
Volume6
DOIs
Publication statusPublished - 2015 Feb

Bibliographical note

Publisher Copyright:
© 2014 Macmillan Publishers Limited. All rights reserved.

All Science Journal Classification (ASJC) codes

  • General
  • General Physics and Astronomy
  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology

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