A Dodecapeptide Selected by Phage Display as a Potential Theranostic Probe for Colon Cancers

Moon Hwa Kwak, Gawon Yi, Seung Mok Yang, Younghee Choe, Sangkee Choi, Hye soo Lee, Eunha Kim, Yong beom Lim, Kun Na, Myung Gyu Choi, Heebeom Koo, Jae Myung Park

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Aim: Colon cancer is one of the leading causes of cancer-related mortality. However, specific biomarkers for its diagnosis or treatment are not established well. Methods: We developed a colon-cancer specific peptide probe using phage display libraries. We validated the specificity of this probe to colon cancer cells with immunohistochemical staining and FACS analysis using one normal cell and five colon cancer cell lines. Results: This peptide probe maintained binding affinity even after serum incubation. For therapeutic applications, this peptide probe was conjugated to hematoporphyrin, a photosensitizer, which showed a significantly enhanced cellular uptake and high photodynamic effect to kill tumor cells. As another application, we made a nanoparticle modified from the peptide probe. It efficiently delivered SN-38, an anticancer drug, into tumor cells, and its tumor-targeting ability was observed in vivo after intravenous injection to the same xenograft model. Conclusion: The noble dodecapeptide probe can be a promising candidate for both colon tumor diagnosis and targeted drug delivery.

Original languageEnglish
Article number100798
JournalTranslational Oncology
Volume13
Issue number9
DOIs
Publication statusPublished - 2020 Sept

Bibliographical note

Publisher Copyright:
© 2020 The Authors

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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