A comparison of Ypet and firefly luciferase as reporter proteins for high-throughput screening

Sei Mee Yoon; Wan Namkung; Jinu Lee

doi:10.1271/bbb.130537

A comparison of Ypet and firefly luciferase as reporter proteins for high-throughput screening

Sei Mee Yoon, Wan Namkung, Jinu Lee

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

When Ypet was used as a reporter protein for highthroughput screening (HTS), it showed peak fold induction and a dynamic range similar to those for firefly luciferase. We also determined that conducting a reading immediately after media aspiration was the best method for HTS. We conclude that Ypet can serve as a substitute for luciferase as a reporter protein in HTS assays.

Original language	English
Pages (from-to)	2328-2330
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	77
Issue number	11
DOIs	https://doi.org/10.1271/bbb.130537
Publication status	Published - 2013

Bibliographical note

Funding Information:
We thank Dr. Klaus Hahn of the University of North Carolina at Chapel Hill for the pYpet-C1 plasmid. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (NRF-2011-0023701).

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.130537

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abstract = "When Ypet was used as a reporter protein for highthroughput screening (HTS), it showed peak fold induction and a dynamic range similar to those for firefly luciferase. We also determined that conducting a reading immediately after media aspiration was the best method for HTS. We conclude that Ypet can serve as a substitute for luciferase as a reporter protein in HTS assays.",

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note = "Funding Information: We thank Dr. Klaus Hahn of the University of North Carolina at Chapel Hill for the pYpet-C1 plasmid. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (NRF-2011-0023701).",

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AB - When Ypet was used as a reporter protein for highthroughput screening (HTS), it showed peak fold induction and a dynamic range similar to those for firefly luciferase. We also determined that conducting a reading immediately after media aspiration was the best method for HTS. We conclude that Ypet can serve as a substitute for luciferase as a reporter protein in HTS assays.

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A comparison of Ypet and firefly luciferase as reporter proteins for high-throughput screening

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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