Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca 2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.
Bibliographical noteFunding Information:
We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.
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