A compact light-sheet microscope for the study of the mammalian central nervous system

Zhengyi Yang; Peter Haslehurst; Suzanne Scott; Nigel Emptage; Kishan Dholakia

doi:10.1038/srep26317

A compact light-sheet microscope for the study of the mammalian central nervous system

Zhengyi Yang, Peter Haslehurst, Suzanne Scott, Nigel Emptage, Kishan Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca 2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.

Original language	English
Article number	26317
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep26317
Publication status	Published - 2016 May 24

Bibliographical note

Funding Information:
We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.

All Science Journal Classification (ASJC) codes

General

Access to Document

10.1038/srep26317

Cite this

@article{050c65d8a5274a46a9b19f22ec2367e9,

title = "A compact light-sheet microscope for the study of the mammalian central nervous system",

abstract = "Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca 2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.",

author = "Zhengyi Yang and Peter Haslehurst and Suzanne Scott and Nigel Emptage and Kishan Dholakia",

note = "Funding Information: We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.",

year = "2016",

month = may,

day = "24",

doi = "10.1038/srep26317",

language = "English",

volume = "6",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - A compact light-sheet microscope for the study of the mammalian central nervous system

AU - Yang, Zhengyi

AU - Haslehurst, Peter

AU - Scott, Suzanne

AU - Emptage, Nigel

AU - Dholakia, Kishan

N1 - Funding Information: We thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Biotechnology and Biological Sciences Research Council (BBSRC) and the Wellcome Trust for funding.

PY - 2016/5/24

Y1 - 2016/5/24

N2 - Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca 2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.

AB - Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca 2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.

UR - http://www.scopus.com/inward/record.url?scp=84971351690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84971351690&partnerID=8YFLogxK

U2 - 10.1038/srep26317

DO - 10.1038/srep26317

M3 - Article

C2 - 27215692

AN - SCOPUS:84971351690

SN - 2045-2322

VL - 6

JO - Scientific reports

JF - Scientific reports

M1 - 26317

ER -

A compact light-sheet microscope for the study of the mammalian central nervous system

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this