A coil-to-helix transition serves as a binding motif for hSNF5 and BAF155 interaction

Jeongmin Han, Iktae Kim, Jae Hyun Park, Ji Hye Yun, Keehyoung Joo, Taehee Kim, Gye Young Park, Kyoung Seok Ryu, Yoon Joo Ko, Kenji Mizutani, Sam Young Park, Rho Hyun Seong, Jooyoung Lee, Jeong Yong Suh, Weontae Lee

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Human SNF5 and BAF155 constitute the core subunit of multi-protein SWI/SNF chromatin-remodeling complexes that are required for ATP-dependent nucleosome mobility and transcriptional control. Human SNF5 (hSNF5) utilizes its repeat 1 (RPT1) domain to associate with the SWIRM domain of BAF155. Here, we employed X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and various biophysical methods in order to investigate the detailed binding mechanism between hSNF5 and BAF155. Multi-angle light scattering data clearly indicate that hSNF5171–258 and BAF155SWIRM are both monomeric in solution and they form a heterodimer. NMR data and crystal structure of the hSNF5171–258 /BAF155SWIRM complex further reveal a unique binding interface, which involves a coil-to-helix transition upon protein binding. The newly formed αN helix of hSNF5171–258 interacts with the β2–α1 loop of hSNF5 via hydrogen bonds and it also displays a hydrophobic interaction with BAF155SWIRM. Therefore, the N-terminal region of hSNF5171–258 plays an important role in tumorigenesis and our data will provide a structural clue for the pathogenesis of Rhabdoid tumors and malignant melanomas that originate from mutations in the N-terminal loop region of hSNF5.

Original languageEnglish
Article number2452
JournalInternational journal of molecular sciences
Volume21
Issue number7
DOIs
Publication statusPublished - 2020 Apr 1

Bibliographical note

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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