TY - JOUR
T1 - A carbohydrate antigen of Clonorchis sinensis recognized by a species-specific monoclonal antibody.
AU - Yong, T. S.
AU - Lee, J. S.
AU - Cho, S. N.
AU - Seo, J. H.
AU - Park, H.
PY - 1996/12
Y1 - 1996/12
N2 - The enzyme-linked immunosorbent assay (ELISA)-inhibition test using a Clonorchis sinensis species-specific mouse monoclonal antibody (MAb), CsHyb 0605-23, showed increased specificity over the conventional ELISA used for serodiagnosis of clonorchiasis. To characterize the corresponding antigen further, the MAb was tested against polysaccharide, protein and glycolipid fractions obtained from a crude extract of C. sinensis adult worms, using chloroform, methanol and phenol extractions. Only the polysaccharide fraction was recognized by the MAb among those fractions. Mild oxidation of the antigen with sodium periodate showed decreased reactivity against the MAb. We concluded that the antigen and antigenic determinants recognized by the MAb are carbohydrates.
AB - The enzyme-linked immunosorbent assay (ELISA)-inhibition test using a Clonorchis sinensis species-specific mouse monoclonal antibody (MAb), CsHyb 0605-23, showed increased specificity over the conventional ELISA used for serodiagnosis of clonorchiasis. To characterize the corresponding antigen further, the MAb was tested against polysaccharide, protein and glycolipid fractions obtained from a crude extract of C. sinensis adult worms, using chloroform, methanol and phenol extractions. Only the polysaccharide fraction was recognized by the MAb among those fractions. Mild oxidation of the antigen with sodium periodate showed decreased reactivity against the MAb. We concluded that the antigen and antigenic determinants recognized by the MAb are carbohydrates.
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U2 - 10.3347/kjp.1996.34.4.279
DO - 10.3347/kjp.1996.34.4.279
M3 - Article
C2 - 9017915
AN - SCOPUS:0030334065
SN - 0023-4001
VL - 34
SP - 279
EP - 281
JO - The Korean journal of parasitology
JF - The Korean journal of parasitology
IS - 4
ER -
