3D-printed graphene direct electron transfer enzyme biosensors

Adaris M. López Marzo, Carmen C. Mayorga-Martinez, Martin Pumera

Research output: Contribution to journalArticlepeer-review

114 Citations (Scopus)


Three-dimensional (3D) printing technology offers attractive possibilities for many fields. In electrochemistry, 3D printing technology has been used to fabricate customized 3D-printed electrodes as a platform to develop bio/sensing, energy generation and storage devices. Here, we use a 3D-printed graphene/polylactic (PLA) electrode made by additive manufacturing technology and immobilize horseradish peroxidase (HRP) to create a direct electron transfer enzyme-based biosensors for hydrogen peroxide detection. Gold nanoparticles are included in the system to confirm and facilitate heterogeneous electron transfer. This work opens a new direction for the fabrication of third-generation electrochemical biosensors using 3D printing technology, with implications for applications in the environmental and biomedical fields.

Original languageEnglish
Article number111980
JournalBiosensors and Bioelectronics
Publication statusPublished - 2020 Mar 1

Bibliographical note

Publisher Copyright:
© 2019

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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