3,3'-diethylthiatricarbocyanine iodide: A highly sensitive chiroptical reporter of DNA helicity and sequence

Jung Kyu Choi; Alessandro D'Urso; Mitch Trauernicht; Murtaza Shabbir-Hussain; Andrea E. Holmes; Milan Balaz

doi:10.3390/ijms12118052

3,3'-diethylthiatricarbocyanine iodide: A highly sensitive chiroptical reporter of DNA helicity and sequence

Jung Kyu Choi, Alessandro D'Urso, Mitch Trauernicht, Murtaza Shabbir-Hussain, Andrea E. Holmes, Milan Balaz

Integrated Sciences and Engineering Division

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3'-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)2 and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.

Original language	English
Pages (from-to)	8052-8062
Number of pages	11
Journal	International journal of molecular sciences
Volume	12
Issue number	11
DOIs	https://doi.org/10.3390/ijms12118052
Publication status	Published - 2011 Nov

All Science Journal Classification (ASJC) codes

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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abstract = "Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3'-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)2 and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.",

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AU - Choi, Jung Kyu

AU - D'Urso, Alessandro

AU - Trauernicht, Mitch

AU - Shabbir-Hussain, Murtaza

AU - Holmes, Andrea E.

AU - Balaz, Milan

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3,3'-diethylthiatricarbocyanine iodide: A highly sensitive chiroptical reporter of DNA helicity and sequence

Abstract

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